On November 28, 2015, Deen was accused on Twitter of rape by the pornographic actress and writer Stoya. In a tweet, Stoya stated that Deen sexually penetrated her through the use of coercion and without her consent: James Deen held me down and fucked me while I said no, stop, used my safeword. I just can't nod and smile when people bring him up anymore.
Deen's former girlfriend Joanna Angel was one of the first performers to tweet support for Stoya. On The Jason Ellis Show, she accused Deen of being abusive during their six-year relationship. Adult performer Sydney Leathers stated that Angel warned her about Deen having "boundary issues" and trying to "break women." Deen's history of rape jokes on social media has also been called into question.
Former adult performer Tori Lux alleged that Deen physically attacked her while working on an adult film in 2011, striking her "five or six times" in the face with his hand after she had explicitly said "no" to him.
Ashley Fires alleged Deen pushed his erection "into my butt" before she said no on a Kink.com set, and that he later confronted her to tell her to stop talking about the incident. Deen is the only performer she refuses to work with because of this incident.
Amber Rayne recounted the time Deen had punched and injured her on set even though they became friends years later.
Kora Peters alleged that Deen violated her anally during a scene where it was not scripted and that the crew congratulated him afterwards.
Nicki Blue alleged that Deen had urinated in her mouth and, along with Princess Donna, sexually assaulted her with a beer bottle when she was staying at Kink.com headquarters.
Lily LaBeau told Vocativ that during a Kink shoot, Deen attempted to use a cattle prod that was on LaBeau's list of prohibited acts and eventually slapped her so hard her jaw locked. Derrick Pierce, a co-star, corroborated LaBeau's account. LaBeau continued to work with Deen after this shoot because she felt she had to for her career.
Farrah Abraham said that she was drugged and raped by Deen
Miss Genocide claimed Deen was abusive on set and during one scene choked her to the point where she briefly lost consciousness.
Bree Olson called Deen a "sadist" and said he was unnecessarily rough during scenes, penetrating her violently and even causing her to cry during one scene.